Integrative vectors for regulated expression of SARS-CoV-2 proteins implicated in RNA metabolism.
Bresson S, Robertson N, Sani E, Turowski TW, Shchepachev V, Kompauerova M, Spanos C, Helwak A, Tollervey D
Wellcome Open Res. 2020 Nov 3;5:261. doi: 10.12688/wellcomeopenres.16322.1. eCollection 2020.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157677 | pcDNA5-FRT-TO-N-Flag-8His | N-terminal tagging vector |
| 157678 | pcDNA5-FRT-TO-C-8His-Flag | C-terminal tagging vector |
| 157679 | pcDNA5-FRT-TO-Nsp1 | mammalian expression of untagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter |
| 157680 | pcDNA5-FRT-TO-FH-Nsp1 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter |
| 157681 | pcDNA5-FRT-TO-Nsp1-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter |
| 157682 | pcDNA5-FRT-TO-Nsp2 | mammalian expression of untagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter |
| 157683 | pcDNA5-FRT-TO-FH-Nsp2 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter |
| 157684 | pcDNA5-FRT-TO-Nsp2-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter |
| 157685 | pcDNA5-FRT-TO-Nsp5 | mammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter |
| 157686 | pcDNA5-FRT-TO-FH-Nsp5 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter |
| 157687 | pcDNA5-FRT-TO-Nsp5-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter |
| 157688 | pcDNA5-FRT-TO-Nsp7 | mammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter |
| 157689 | pcDNA5-FRT-TO-FH-Nsp7 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter |
| 157690 | pcDNA5-FRT-TO-Nsp7-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter |
| 157691 | pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt | mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter |
| 157692 | pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt | mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter |
| 157693 | pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt | mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter |
| 157694 | pcDNA5-FRT-TO-Nsp8 | mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter |
| 157695 | pcDNA5-FRT-TO-FH-Nsp8 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter |
| 157696 | pcDNA5-FRT-TO-Nsp8-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter |
| 157697 | pcDNA5-FRT-TO-Nsp8_GC3opt | mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter |
| 157698 | pcDNA5-FRT-TO-FH-Nsp8_GC3opt | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter |
| 157699 | pcDNA5-FRT-TO-Nsp8-HF_GC3opt | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter |
| 157700 | pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt | mammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter |
| 157701 | pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter |
| 157702 | pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter |
| 157703 | pcDNA5-FRT-TO-Nsp9 | mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter |
| 157704 | pcDNA5-FRT-TO-FH-Nsp9 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter |
| 157705 | pcDNA5-FRT-TO-Nsp9-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter |
| 157706 | pcDNA5-FRT-TO-Nsp10 | mammalian expression of untagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter |
| 157707 | pcDNA5-FRT-TO-FH-Nsp10 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter |
| 157708 | pcDNA5-FRT-TO-Nsp10-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter |
| 157709 | pcDNA5-FRT-TO-Nsp12 | mammalian expression of untagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter |
| 157710 | pcDNA5-FRT-TO-FH-Nsp12 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter |
| 157711 | pcDNA5-FRT-TO-Nsp12-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter |
| 157712 | pcDNA5-FRT-TO-Nsp13 | mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter |
| 157713 | pcDNA5-FRT-TO-FH-Nsp13 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter |
| 157714 | pcDNA5-FRT-TO-Nsp13_GC3opt | mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter |
| 157715 | pcDNA5-FRT-TO-FH-Nsp13_GC3opt | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter |
| 157716 | pcDNA5-FRT-TO-Nsp13-HF_GC3opt | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter |
| 157717 | pcDNA5-FRT-TO-Nsp14 | mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter |
| 157718 | pcDNA5-FRT-TO-FH-Nsp14 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter |
| 157719 | pcDNA5-FRT-TO-Nsp14-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter |
| 157720 | pcDNA5-FRT-TO-Nsp15 | mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter |
| 157721 | pcDNA5-FRT-TO-FH-Nsp15 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter |
| 157722 | pcDNA5-FRT-TO-Nsp15-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter |
| 157723 | pcDNA5-FRT-TO-Nsp16 | mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter |
| 157724 | pcDNA5-FRT-TO-FH-Nsp16 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter |
| 157725 | pcDNA5-FRT-TO-Nsp16-HF | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter |
| 157726 | pcDNA5-FRT-TO-Orf6 | mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter |
| 157727 | pcDNA5-FRT-TO-FH-Orf6 | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter |
| 157728 | pcDNA5-FRT-TO-Nprot | mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter |
| 157729 | pcDNA5-FRT-TO-FH-Nprot | mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter |
| 157730 | pcDNA-FRT-TO-Nprot_GC3opt | mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter |
| 157732 | pcDNA5-FRT-TO-Nprot-HF_GC3opt | mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter |
| 157733 | pcDNA5-FRT-TO-eGFP | mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter |
| 157734 | pcDNA5-FRT-TO-FH-eGFP | mammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter |
| 157735 | pcDNA5-FRT-TO-eGFP-HF | mammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoter |