A Split Methyl Halide Transferase AND Gate That Reports by Synthesizing an Indicator Gas.
Fulk EM, Huh D, Atkinson JT, Lie M, Masiello CA, Silberg JJ
ACS Synth Biol. 2020 Nov 20;9(11):3104-3113. doi: 10.1021/acssynbio.0c00355. Epub 2020 Oct 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157631 | pEMF034 | sMHT-113 |
| 157632 | pEMF035 | sMHT-67 |
| 157633 | pEMF036 | sMHT-129 |
| 157634 | pEMF037 | Batis maritima MHT |
| 157635 | pEMF038 | Empty vector |
| 157636 | pEMF047 | sMHT-113 F1 only |
| 157637 | pEMF044 | sMHT-113 F2 only |
| 157638 | pDH013 | sMHT-113 fused to GFP and RFP |
| 157639 | pDH001 | Batis maritima MHT |
| 157640 | pDH003 | sMHT-113 fused to SYNZIP17/18 |
| 157641 | pDH006 | sMHT-113 fused SYNZIP18 only |
| 157642 | pDH007 | sMHT-113 fused to CheA and CheY |
| 157643 | pDH008 | sMHT-113 fused to CheY only |
| 157644 | pDH009 | sMHT-113 fused to GRX2 |
| 157645 | pDH010 | sMHT-113 fused non-dimerizing GRX2-C37A mutant |
| 157646 | pDH011 | sMHT-113 fused to FKBP/FRB |
| 157647 | pDH012 | Constitutive sMHT-113-SYNZIP expression |