Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs.
Kern DM, Sorum B, Mali SS, Hoel CM, Sridharan S, Remis JP, Toso DB, Kotecha A, Bautista DM, Brohawn SG
bioRxiv [Preprint]. 2021 Jan 26:2020.06.17.156554. doi: 10.1101/2020.06.17.156554.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 156422 | pSBL_DK186 | Mammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells |
| 156423 | pSBL_DK187 | Insect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmid |
| 156424 | pSBL_DK193 | Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid |
| 156425 | pSBL_DK198 | Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid |
| 156426 | pSBL_DK202 | Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells |