SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity.

Zhang L, Jackson CB, Mou H, Ojha A, Peng H, Quinlan BD, Rangarajan ES, Pan A, Vanderheiden A, Suthar MS, Li W, Izard T, Rader C, Farzan M, Choe H
Nat Commun. 2020 Nov 26;11(1):6013. doi: 10.1038/s41467-020-19808-4. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
159364	pCAGGS-SARS2-S-FKO (C-flag)	Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.
159363	pCAGGS-SARS2-S-FKO (NC-flag)	Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.
156421	pCAGGS-SARS2-S-G614 (C-flag)	Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
156420	pCAGGS-SARS2-S-D614 (C-flag)	Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
156419	pCAGGS-SARS2-S-G614 (NC-flag)	Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
156418	pCAGGS-SARS2-S-D614 (NC-flag)	Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.