A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo.

Moore CL, Papa LJ 3rd, Shoulders MD
J Am Chem Soc. 2018 Sep 19;140(37):11560-11564. doi: 10.1021/jacs.8b04001. Epub 2018 Jul 18. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
156454	T7 promoter + filler DNA reporter plasmid	A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.
156455	T7 promoter + rpsL reporter plasmid	A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.
156456	T7 promoter + terminators reporter plasmid	A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.
156457	Dual opposing T7 promoters reporter plasmid	A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.
156459	rApoI	An E. coli strain that expresses rat APOBEC1. Used as a control strain for mutagenesis experiments with MutaT7.
156460	MutaT7	An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.
156461	drApo1–T7	An E. coli strain that expresses an inactive variant of MutaT7. Used as a control strain for mutagenesis experiments with MutaT7.