Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas.
Kuo J, Yuan R, Sanchez C, Paulsson J, Silver PA
Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 10.1093/nar/gkaa557.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155369 | pJK384.4 | dCas9 + PA4-mVenus |
| 155370 | pJK398 | dCas9 sgRNA oscillator v1 (dCRv1) (p15A) |
| 155371 | pJK412 | dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] |
| 155372 | pJK413 | dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412) |
| 155373 | pJK422 | dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP) |
| 155374 | pJK423 | dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP) |
| 155376 | pJK429 | PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus] |
| 155377 | pJK430 | PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus] |
| 155378 | pJK434 | PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP) |
| 155380 | pJK503 | dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus |
| 155381 | pJK504 | dCas12a crRNAs |
| 155382 | pJK506 | dCas12a (D917A) + PA4-mVenus. (removed crRNAs) |
| 155384 | pJK511 | dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus |
| 155385 | pJK517 | PphlF-TA4 in crRNAs |
| 155386 | pJK520 | PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus |
| 155387 | pJK526 | PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus |
| 155388 | pJK528 | dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP) |