Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas.

Kuo J, Yuan R, Sanchez C, Paulsson J, Silver PA
Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 10.1093/nar/gkaa557. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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Search table:

ID	Plasmid	Purpose
155388	pJK528	dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
155387	pJK526	PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus
155386	pJK520	PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus
155385	pJK517	PphlF-TA4 in crRNAs
155384	pJK511	dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
155382	pJK506	dCas12a (D917A) + PA4-mVenus. (removed crRNAs)
155381	pJK504	dCas12a crRNAs
155380	pJK503	dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus
155378	pJK434	PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)
155377	pJK430	PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]