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Bottom-up de novo design of functional proteins with complex structural features.

Yang C, Sesterhenn F, Bonet J, van Aalen EA, Scheller L, Abriata LA, Cramer JT, Wen X, Rosset S, Georgeon S, Jardetzky T, Krey T, Fussenegger M, Merkx M, Correia BE
Nat Chem Biol. 2021 Apr;17(4):492-500. doi: 10.1038/s41589-020-00699-x. Epub 2021 Jan 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
155198pET_3H1L_02.395Expresses 3H1L_02.395 in E.coli
1552084E1H.95_LUMABSExpresses 4E1H.95_LUMABS sensor in E.coli
1552094E2H.210_LUMABSExpresses 4E2H.210_LUMABS sensor in E.coli
155210pET_4H.01Expresses 4H.01 de novo designed protein in E.coli
155211pET_3E2H.37Expresses 3E2H.37 de novo designed protein in E.coli
155212pET_4E2H.210Expresses 4E2H.210 de novo protein in E.coli
155213pET_4E1H.95Expresses 4E1H.95 de novo protein in E.coli

Antibodies from Article