Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells.
Chang Y, Syahirah R, Oprescu SN, Wang X, Jung J, Cooper SH, Torregrosa-Allen S, Elzey BD, Hsu AY, Randolph LN, Sun Y, Kuang S, Broxmeyer HE, Deng Q, Lian X, Bao X
Biomaterials. 2022 May 6;285:121569. doi: 10.1016/j.biomaterials.2022.121569.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155182 | XLone-Puro Cas13d P2A eGFP-NLS | PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP |
| 155183 | XLone-BSD Cas13d P2A mCherry | PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry |
| 155184 | XLone-Puro Cas13d-eGFP U6 BbsI | PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone |
| 155185 | XLone-Puro Cas13d-eGFP U6 RUNX1 g1 | PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA1 |
| 155186 | XLone-Puro Cas13d-eGFP U6 RUNX1 g2 | PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA2 |
| 155187 | XLone-Puro Cas13d-eGFP U6 SOX17 g1 | PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA1 |
| 155188 | XLone-Puro Cas13d-eGFP U6 SOX17 g2 | PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2 |
| 155189 | XLone-Puro beta-catenin P2A eGFP-NLS | PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP |
| 159754 | AAVS1-Blasticidin XLone-eGFP | AAVS1 donor plasmid for targeted inducible eGFP expression in human cells |
| 159756 | PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI | PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone |