Plasmid Design for Tunable Two-Enzyme Co-Expression Promotes Whole-Cell Production of Cellobiose.
Schwaiger KN, Voit A, Dobiasova H, Luley C, Wiltschi B, Nidetzky B
Biotechnol J. 2020 Jul 15:e2000063. doi: 10.1002/biot.202000063.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155163 | pBICI_weak | BaSP and CuCBP co-expression: weak RBS controls BaSP expression |
| 155164 | pBICI_medium | BaSP and CuCBP co-expression: medium RBS controls BaSP expression |
| 155165 | pBICI_strong | BaSP and CuCBP co-expression: strong RBS controls BaSP expression |
| 155167 | pBICI_2_medium | see pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site |
| 155168 | pBICI_2_strong | see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site |
| 155169 | pDUAL_lacZ | BaSP and CuCBP co-expression: lacZ promoter controls BaSP expression |
| 155170 | pDUAL_tacI | BaSP and CuCBP co-expression: tacI promoter controls BaSP expression |
| 155172 | pDUAL_araBAD | BaSP and CuCBP co-expression: araBAD promoter controls BaSP expression |
| 155173 | pDUAL_2_lacZ | see pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom site |
| 155174 | pDUAL_2_tacI | see pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site |
| 155175 | pDUAL_2_T5 | see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site |
| 155176 | pDUAL_2_araBAD | see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site |
| 155178 | pBR322-bom | pBICI_2_strong without bom site |