Structure of MlaFB uncovers novel mechanisms of ABC transporter regulation.
Kolich LR, Chang YT, Coudray N, Giacometti SI, MacRae MR, Isom GL, Teran EM, Bhabha G, Ekiert DC
Elife. 2020 Jun 30;9. pii: 60030. doi: 10.7554/eLife.60030.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155138 | pBEL1244 | Expresses MlaFED operon in E. coli |
| 155139 | pBEL1245 | Expresses MlaF in E. coli |
| 155141 | pBEL1246 | Expresses MlaB in E. coli |
| 155142 | pBEL1266 | Expresses MlaF, tagless, araBAD promoter |
| 155143 | pBEL1305 | Expresses MlaFB, His tag on MlaF N-term |
| 155144 | pBEL1306 | Expresses MlaFB, His tag on MlaF C-term |
| 155145 | pBEL1307 | Expresses MlaFB, His tag on MlaB N-term |
| 155146 | pBEL1308 | Expresses MlaFB, His tag on MlaB C-term |
| 155147 | pBEL1514 | Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD |
| 155148 | pBEL1647 | Expresses MlaF 1-246, tagless |
| 155149 | pBEL1833 | Expresses MlaF(1-250)-GCN4 |
| 155150 | pBEL1840 | Expresses MlaF-6xHis |
| 155151 | pBEL1957 | Expresses Strep-MlaF-(6xHis-TEV-MlaB) |
| 155152 | pBEL1965 | Expresses MlaF(1-246)-(6 aa linker)-GCN4 |
| 155153 | pBEL1966 | Expresses MlaF(1-246)-(10 aa linker)-GCN4 |
| 155154 | pBEL1967 | Expresses MlaF(1-246)-(14 aa linker)-GCN4 |
| 155155 | pBEL2074 | Expresses Strep-MlaF-(6xHis-TEV-MlaB_T52E) |
| 155156 | pBEL2076 | Expresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB) |
| 155157 | pBEL2077 | Expresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB) |
| 155158 | pBEL2078 | Expresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB) |
| 155159 | pBEL2079 | Expresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB) |
| 155160 | pBEL2080 | Expresses Strep-MlaF_L266A)-(6xHis-TEV-MlaB) |
| 155161 | pBEL2081 | Expresses (Strep-MlaF_Y261A_L265A_L266A)-(6xHis-TEV-MlaB) |
| 155162 | pBEL2082 | Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB) |