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Structure of MlaFB uncovers novel mechanisms of ABC transporter regulation.

Kolich LR, Chang YT, Coudray N, Giacometti SI, MacRae MR, Isom GL, Teran EM, Bhabha G, Ekiert DC
Elife. 2020 Jun 30;9. pii: 60030. doi: 10.7554/eLife.60030. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
155138pBEL1244Expresses MlaFED operon in E. coli
155139pBEL1245Expresses MlaF in E. coli
155141pBEL1246Expresses MlaB in E. coli
155142pBEL1266Expresses MlaF, tagless, araBAD promoter
155143pBEL1305Expresses MlaFB, His tag on MlaF N-term
155144pBEL1306Expresses MlaFB, His tag on MlaF C-term
155145pBEL1307Expresses MlaFB, His tag on MlaB N-term
155146pBEL1308Expresses MlaFB, His tag on MlaB C-term
155147pBEL1514Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD
155148pBEL1647Expresses MlaF 1-246, tagless
155149pBEL1833Expresses MlaF(1-250)-GCN4
155150pBEL1840Expresses MlaF-6xHis
155151pBEL1957Expresses Strep-MlaF-(6xHis-TEV-MlaB)
155152pBEL1965Expresses MlaF(1-246)-(6 aa linker)-GCN4
155153pBEL1966Expresses MlaF(1-246)-(10 aa linker)-GCN4
155154pBEL1967Expresses MlaF(1-246)-(14 aa linker)-GCN4
155155pBEL2074Expresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)
155156pBEL2076Expresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)
155157pBEL2077Expresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)
155158pBEL2078Expresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)
155159pBEL2079Expresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)
155160pBEL2080Expresses Strep-MlaF_L266A)-(6xHis-TEV-MlaB)
155161pBEL2081Expresses (Strep-MlaF_Y261A_L265A_L266A)-(6xHis-TEV-MlaB)
155162pBEL2082Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

Antibodies from Article