Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.

Aregger M, Lawson KA, Billmann M, Costanzo M, Tong AHY, Chan K, Rahman M, Brown KR, Ross C, Usaj M, Nedyalkova L, Sizova O, Habsid A, Pawling J, Lin ZY, Abdouni H, Wong CJ, Weiss A, Mero P, Dennis JW, Gingras AC, Myers CL, Andrews BJ, Boone C, Moffat J
Nat Metab. 2020 Jun;2(6):499-513. doi: 10.1038/s42255-020-0211-z. Epub 2020 Jun 1. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
155085	pLCKO_AAVS1_sgRNA_2	lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus
155086	pLCKO_AAVS1_sgRNA_3	lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus
155087	pLCKO_AAVS1_sgRNA_4	lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus
155088	LCV2_AAVS1_sgRNA_2	lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus
155089	LCV2_AAVS1_sgRNA_3	lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus
155090	LCV2_AAVS1_sgRNA_4	lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus
155091	LCV2_PSMD1_sgRNA_1	lentiviral plasmid expressing Cas9 and gRNA targeting PSMD1 (core essential gene)
155092	LCV2_PSMB2_sgRNA_1	lentiviral plasmid expressing Cas9 and gRNA targeting PSMB2 (core essential gene)
155093	LCV2_LacZ_sgRNA_2	lentiviral plasmid expressing Cas9 and gRNA targeting LacZ
155094	LCV2_Luciferase_sgRNA	lentiviral plasmid expressing Cas9 and gRNA targeting Luciferase
155095	LCV2_mClover3	Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression
155096	LCV2_mCherry	Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression
155097	LCV2_BFP2	Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression
155098	LCV2_EGFP	Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression
155099	LCV2_mClover3_AAVS1_sgRNA_2	lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus
155101	LCV2_mClover3_AAVS1_sgRNA_4	lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus
155102	LCV2_mClover3_PSMD1_sgRNA_1	lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene)
155103	LCV2_mClover3_LacZ	lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZ
155105	LCV2_mCherry_AAVS1_sgRNA_2	lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus
155106	LCV2_mCherry_AAVS1_sgRNA_4	lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus
155107	LCV2_mCherry_PSMD1_sgRNA_1	lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting PSMD1 (core essential gene)
155108	LCV2_mCherry_LacZ_sgRNA	lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ
155109	LCV2_mCherry_Luciferase_sgRNA	lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting Luciferase
155110	pLX304_C12orf49-V5	lentiviral plasmid for the exogneous expression of C-terminal V5-tagged C12orf49
155111	pCDNA5_miniTurbo-C12orf49	transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID
155112	pCDNA5_C12orf49-miniTurbo	transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID