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Curing cytoplasmic male sterility via TALEN-mediated mitochondrial genome editing.

Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI
Nat Plants. 2019 Jul;5(7):722-730. doi: 10.1038/s41477-019-0459-z. Epub 2019 Jul 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
158726pDESTpK7WG2mitoPlant mitoTALEN vector assembly, Destination vector
158727pENTR E2 HSPter-35Sp-mitoPlant mitoTALEN vector assembly, Entry clone 2 with attL1 L4
158728pENTR E1 pF5A L1-L4Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last HD)
158729pENTR E1 pF6A L1-L4Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NG)
158730pENTR E1 pF7A L1-L4Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NI)
158731pENTR E1 pF8A L1-L4Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NN)
158732pENTR E3 pR5A L3-L2Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)
158733pENTR E3 pR6A L3-L2Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NG)
158734pENTR E3 pR7A L3-L2Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NI)
158735pENTR E3 pR8A L3-L2Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NN)
158736pK7WG2-mitoTAL1-orf125Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138)
158737pK7WG2-mitoTAL2-orf125Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138)

Antibodies from Article