CRISPR-Cas "Non-Target" Sites Inhibit On-Target Cutting Rates.
Moreb EA, Hutmacher M, Lynch MD
CRISPR J. 2020 Dec;3(6):550-561. doi: 10.1089/crispr.2020.0065.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154919 | pKDsgRNA-pgaC | aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes |
| 154920 | pKDsgRNA-pheT | aTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymes |
| 154921 | pKDsgRNA-mqo | aTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymes |
| 154922 | pKDsgRNA-hisF | aTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes |
| 154923 | pKDsgRNA-ampG | aTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymes |
| 154924 | pEM-Cas9HF1-D1135E | aTc inducible Cas9HF1 with D1135E mutation |
| 154925 | pEM-eCas9 | aTc inducible eCas9 |
| 154926 | pEM-eCas9-D1135E | aTc inducible eCas9 with D1135E mutation |
| 154927 | pEM-OptiCas9 | aTc inducible OptiCas9 |
| 154928 | pEM-OptiCas9-D1135E | aTc inducible OptiCas9 with D1135E mutation |
| 154929 | pEM-HypaCas9 | aTc inducible HypaCas9 |
| 154930 | pEM-HypaCas9-D1135E | aTc inducible HypaCas9 with D1135E mutation |
| 154931 | pEM-Cas9-D1135E | aTc inducible Cas9 with D1135E mutation |
| 154932 | pNT-15 | 15 designed non-target sites |
| 154933 | pNT-12 | 12 designed non-target sites |
| 154934 | pNT-3 | 3 designed non-target sites |
| 154935 | pNT-1 | 1 designed non-target site |