A viral toolkit for recording transcription factor-DNA interactions in live mouse tissues.
Cammack AJ, Moudgil A, Chen J, Vasek MJ, Shabsovich M, McCullough K, Yen A, Lagunas T, Maloney SE, He J, Chen X, Hooda M, Wilkinson MN, Miller TM, Mitra RD, Dougherty JD
Proc Natl Acad Sci U S A. 2020 May 5;117(18):10003-10014. doi: 10.1073/pnas.1918241117. Epub 2020 Apr 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86949 | pAAV-short Cre-on G/W | Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAV |
| 86950 | AAV-short_BrokenHeart (no BC) | AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence. |
| 86954 | pAAV_GWRev_Dest | Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off) |
| 86955 | pAAV_GW_Dest | Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter. |
| 154889 | AAV-PB-SRT-tdTomato | piggyBac self-reporting transposon with tdTomato marker in AAV backbone |