Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.
Ponsford AH, Ryan TA, Raimondi A, Cocucci E, Wycislo SA, Frohlich F, Swan LE, Stagi M
Autophagy. 2020 Jun 9:1-19. doi: 10.1080/15548627.2020.1771858.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157940 | pCAG R2pH-LAMP1-3xFLAG | expresses ratiometric sensor of lysosomal lumenal pH |
| 157941 | pDONOR223 R2pH-LAMP1-3xFLAG | gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH |
| 163018 | pCAG RpH-LAMP1-3xFLAG | expresses ratiometric sensor of lysosomal lumenal pH |
| 163019 | pDONOR223 RpH-LAMP1-3xFLAG | gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH |