Designed Parasite-Selective Rhomboid Inhibitors Block Invasion and Clear Blood-Stage Malaria.
Gandhi S, Baker RP, Cho S, Stanchev S, Strisovsky K, Urban S
Cell Chem Biol. 2020 Aug 31. pii: S2451-9456(20)30333-0. doi: 10.1016/j.chembiol.2020.08.011.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154858 | pcDNA-HA-PfROM4 (H578A) | Expresses N-terminally HA-tagged PfROM4 variant H578A (codon optimized for mammalian expression) from pcDNA3.1 |
| 154859 | pcDNA-HA-PfROM4 (G470A/G471N) | Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1 |
| 154873 | pcDNA-HA-PfROM1 (A144G/N145G) | Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1 |
| 154875 | pcDNA-GFP-BAEBL (I1134V/P1135L/Y1136V/F1137V) | Expresses N-terminally GFP-tagged Pf-BAEBL P5-P2 VLVV variant (coding for residues 1041-1210) from pcDNA3.1 |
| 154970 | pcDNA-GFP-EBA175-Flag | Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag. |
| 154971 | pcDNA-GFP-EBA175-Flag(P1424A) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A variant (residues 1284-1462) from pcDNA3.1 |
| 154972 | pcDNA-GFP-EBA175-Flag(P1424I) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424I variant (residues 1284-1462) from pcDNA3.1 |
| 154973 | pcDNA-GFP-EBA175-Flag(Y1425A) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1425A variant (residues 1284-1462) from pcDNA3.1 |
| 154974 | pcDNA-GFP-EBA175-Flag(Y1425I) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1425I variant (residues 1284-1462) from pcDNA3.1 |
| 154975 | pcDNA-GFP-EBA175-Flag(Y1426A) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426A variant (residues 1284-1462) from pcDNA3.1 |
| 154976 | pcDNA-GFP-EBA175-Flag(Y1426I) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426I variant (residues 1284-1462) from pcDNA3.1 |
| 154977 | pcDNA-GFP-EBA175-Flag(P1424I/Y1425I/Y1426I) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A/Y1425I/Y1426I variant (residues 1284-1462) from pcDNA3.1 |
| 154978 | pcDNA-GFP-EBA175-Flag(P1424C) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424C variant (residues 1284-1462) from pcDNA3.1 |
| 154979 | pcDNA-GFP-EBA175-Flag(P1424M) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424M variant (residues 1284-1462) from pcDNA3.1 |
| 154988 | pcDNA-GFP-EBA175-Flag(P1424V) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V variant (residues 1284-1462) from pcDNA3.1 |
| 154989 | pcDNA-GFP-EBA175-Flag(P1424L) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424L variant (residues 1284-1462) from pcDNA3.1 |
| 154990 | pcDNA-GFP-EBA175-Flag(P1424F) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424F variant (residues 1284-1462) from pcDNA3.1 |
| 154991 | pcDNA-GFP-EBA175-Flag(P1424Y) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424Y variant (residues 1284-1462) from pcDNA3.1 |
| 154992 | pcDNA-GFP-EBA175-Flag(P1424W) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424W variant (residues 1284-1462) from pcDNA3.1 |
| 154993 | pcDNA-GFP-EBA175-Flag(P1424T) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424T variant (residues 1284-1462) from pcDNA3.1 |
| 154994 | pcDNA-GFP-EBA175-Flag(P1424S) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424S variant (residues 1284-1462) from pcDNA3.1 |
| 154995 | pcDNA-GFP-EBA175-Flag(P1424N) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424N variant (residues 1284-1462) from pcDNA3.1 |
| 154996 | pcDNA-GFP-EBA175-Flag(P1424Q) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424Q variant (residues 1284-1462) from pcDNA3.1 |
| 154997 | pcDNA-GFP-EBA175-Flag(P1424R) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424R variant (residues 1284-1462) from pcDNA3.1 |
| 154998 | pcDNA-GFP-EBA175-Flag(P1424K) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424K variant (residues 1284-1462) from pcDNA3.1 |
| 154999 | pcDNA-GFP-EBA175-Flag(P1424H) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424H variant (residues 1284-1462) from pcDNA3.1 |
| 155000 | pcDNA-GFP-EBA175-Flag(P1424D) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424D variant (residues 1284-1462) from pcDNA3.1 |
| 155001 | pcDNA-GFP-EBA175-Flag(P1424E) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424E variant (residues 1284-1462) from pcDNA3.1 |
| 155003 | pcDNA-GFP-EBA175-Flag(P1424Q/Y1426S) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1 |
| 155004 | pcDNA-GFP-EBA175-Flag(P1424R/Y1426S) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424R/Y1426S variant (residues 1284-1462) from pcDNA3.1 |
| 155005 | pcDNA-GFP-EBA175-Flag(P1424D/Y1426S) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424D/Y1426S variant (residues 1284-1462) from pcDNA3.1 |
| 155006 | pcDNA-GFP-EBA175-Flag(E1418K/P1424Q/Y1426S) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1 |
| 155007 | pcDNA-GFP-EBA175-Flag(A1419R/P1424Q/Y1426S) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1 |
| 155008 | pcDNA-GFP-EBA175-Flag(S1421R/P1424Q/Y1426S) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1 |
| 155009 | pcDNA-GFP-EBA175-Flag(E1418K/A1419R/P1424Q/Y1426S) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1 |
| 155010 | pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1 |
| 155012 | pcDNA-GFP-EphrinB3-Flag | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1 |
| 155013 | pcDNA-GFP-EphrinB3-Flag(A227Y) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1 |
| 155035 | pcDNA-GFP-EBA175-Flag(Y1426S) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426S variant (residues 1284-1462) from pcDNA3.1 |
| 155128 | pcDNA-GFP-EBA175-Flag(P1424G) | Expresses N-terminally GFP-tagged EBA175 P1424G variant (residues 1284-1462) from pcDNA3.1 |
| 155308 | pcDNA-GFP-AMA1-Flag(K546M/I547P/I548Y/I549Y) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged AMA1 K546M/I547P/I548Y/I549Y variant |
| 155309 | pcDNA-GFP-EBA175-Flag(M1423R/P1424V) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V variant (residues 1284-1462) from pcDNA3.1 |
| 155310 | pcDNA-GFP-EBA175-Flag(P1424V/Y1425W) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1425W variant (residues 1284-1462) from pcDNA3.1 |
| 155311 | pcDNA-GFP-EBA175-Flag(P1424V/Y1426H) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1 |
| 155312 | pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1426H) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1 |
| 155313 | pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1425W/Y1426H) | Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1425W/Y1426H variant (residues 1284-1462) from pcDNA3.1 |