Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping.
Hollerer S, Papaxanthos L, Gumpinger AC, Fischer K, Beisel C, Borgwardt K, Benenson Y, Jeschek M
Nat Commun. 2020 Jul 15;11(1):3551. doi: 10.1038/s41467-020-17222-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154842 | pASPIre1 | Derivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sites |
| 154843 | pASPIre2 | Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1 |
| 154844 | pASPIre3 | Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study |