Expanding the Chemogenetic Toolbox by Circular Permutation.
Lee YT, He L, Zhou Y
J Mol Biol. 2020 May 1;432(10):3127-3136. doi: 10.1016/j.jmb.2020.03.033. Epub 2020 Apr 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155389 | mCh-cpFRB1 | Express cpFRB1 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2 |
| 159645 | mCh-cpFRB2 | Express cpFRB2 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2 |
| 165026 | GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C) | Express cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP |
| 165028 | GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C) | Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP |
| 165029 | mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C) | Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry |
| 165030 | mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C) | Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry |