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Congenital disorder of glycosylation caused by starting site-specific variant in syntaxin-5.

Linders PTA, Gerretsen ECF, Ashikov A, Vals MA, de Boer R, Revelo NH, Arts R, Baerenfaenger M, Zijlstra F, Huijben K, Raymond K, Muru K, Fjodorova O, Pajusalu S, Ounap K, Ter Beest M, Lefeber D, van den Bogaart G
Nat Commun. 2021 Oct 28;12(1):6227. doi: 10.1038/s41467-021-26534-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
154845str-KDEL_Stx5S-SBP-mCitrineSynchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)
154846str-KDEL_Stx5LdeltaER-SBP-mCitrineSynchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)
165082pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1gRNA 1 of pair B for Stx5L-specific knockout
165083pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2gRNA 2 of pair B for Stx5L-specific knockout
165085pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1gRNA 1 of pair C for Stx5L-specific knockout
165086pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2gRNA 2 of pair C for Stx5L-specific knockout
165087Stx5S-mCherryExpresses Stx5S-mCherry in mammalian cells
209844Stx5L-mCherryExpresses Stx5L-mCherry in mammalian cells
209845Stx5L(m55V)-GFPExpresses Stx5 M55V mutant fused to GFP in mammalian cells
209846str-KDEL_Stx5L(M55V)-SBP-mCitrineSynchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)
209847Stx5S-GFPExpresses Stx5S-GFP in mammalian cells
209848Bet1-mCherryExpresses Bet1-mCherry in mammalian cells
209849Bet1L-mCherryExpresses Bet1L-mCherry in mammalian cells
209850Bet1-GFPExpresses Bet1-GFP in mammalian cells

Antibodies from Article