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Efficient DNA double-strand break formation at single or multiple defined sites in the Saccharomyces cerevisiae genome.

Gnugge R, Symington LS
Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
154813pRG645_lexO-AscI_LexA-TF_LEU2MXInducible expression of AscI endonuclease in S. cerevisiae
154815pRG646_lexO-HO_LexA-TF_LEU2MXInducible expression of HO endonuclease in S. cerevisiae
154816pRG659_lexO-FseI_LexA-TF_LEU2MXInducible expression of FseI endonuclease in S. cerevisiae
154817pRG705_lexO-AsiSI_LexA-TF_LEU2MXInducible expression of AsiSI endonuclease in S. cerevisiae
154818pRG706_lexO-SbfI_LexA-TF_LEU2MXInducible expression of SbfI endonuclease in S. cerevisiae
154819pRG710_lexO-HO_LexA-TF_HIS3MXInducible expression of HO endonuclease in S. cerevisiae
154820pRG711_lexO-AscI_LexA-TF_HIS3MXInducible expression of AscI endonuclease in S. cerevisiae
154821pRG713_lexO-HO_LexA-TF_URA3MXInducible expression of HO endonuclease in S. cerevisiae
154822pRG714_lexO-AscI_LexA-TF_URA3MXInducible expression of AscI endonuclease in S. cerevisiae

Antibodies from Article