Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells.
Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ
Cell Rep. 2020 Jun 23;31(12):107811. doi: 10.1016/j.celrep.2020.107811.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154763 | pAS_FLAG-G1 PylRS | plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration |
| 154767 | pAS_4xG1 PylT sfGFP 150TAG | plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration |
| 154768 | pAS_4xG1 PylT FLAG-G1 PylRS | plasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration |
| 154769 | pAS_4xG1 PylT FLAG-G1 PylRS Y125A | plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration |
| 154770 | pAS_4xG1 PylT A41AA C55A sfGFP 150TAG | plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration |
| 154771 | pAS_4xhyb PylT sfGFP 150TAG | plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration |
| 154772 | pAS_4xhyb PylT A41AA C55A sfGFP 150TAG | plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration |
| 154773 | pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A | plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration |
| 154774 | pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS | plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration |
| 154775 | pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA | plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration |
| 154776 | pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA | plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration |
| 154777 | pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG | plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration |
| 154778 | pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG | plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration |
| 154779 | pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA | plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration |
| 154780 | pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA | plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration |
| 154781 | pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA | plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration |
| 154783 | pUC_h7SK Mma PylT (UUA) | plasmid with single copy of Mma PylT (UUA anticodon) |
| 154784 | pUC_h7SK M15 (CUA) | plasmid with single copy of Mma PylT M15 mutant (CUA anticodon) |
| 154785 | pUC_h7SK M15 (UUA) | plasmid with single copy of Mma PylT M15 mutant (UUA anticodon) |