An improved molecular tool for screening bacterial colonies using GFP expression enhanced by a Dictyostelium sequence.
Kondo T, Yumura S
Biotechniques. 2020 Feb;68(2):91-95. doi: 10.2144/btn-2019-0127. Epub 2019 Dec 11.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154295 | pUC19/mlcR25-mEGFP | Fluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP) |
| 154297 | pMYU1A-TED | A shuttle vector between E. coli and magnetotactic bacteria (e.g. Magnetospirillum) containing a TED module |
| 154298 | pDYU1G-TED | A expression vector in Dictyostelium discoideum containing the G418 resistant gene and the TED module for GFP-negative screening |
| 154299 | pDYU1B-TED | A expression vector in Dictyostelium discoideum containing the Blasticidin S resistant gene and the TED module for GFP-negative screening |
| 154300 | pDYU1H-TED | A expression vector in Dictyostelium discoideum containing the Hygromycin B resistant gene and the TED module for GFP-negative screening |
| 154301 | pDYU1G/γ-tubulin-mEGFP | Expresses γ-tubulin–mEGFP from actin15 promoter in Dictyostelium discoideum |
| 154302 | pDYU1B/mRuby3–α-tubulin | Expresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideum |
| 154303 | pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP | Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum |
| 154304 | pUC19/DdmEGFP | cloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideum |
| 154305 | pUC19/DdmRuby3 | cloned with the codon-optimized mRuby3 gene for expression in Dictyostelium discoideum |