O-GlcNAc Transferase Recognizes Protein Substrates Using an Asparagine Ladder in the Tetratricopeptide Repeat (TPR) Superhelix.
Levine ZG, Fan C, Melicher MS, Orman M, Benjamin T, Walker S
J Am Chem Soc. 2018 Mar 14;140(10):3510-3513. doi: 10.1021/jacs.7b13546. Epub 2018 Mar 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154280 | pBJG1 T7-8xHis-OGT 5N5A | Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site. |