How the glycosyltransferase OGT catalyzes amide bond cleavage.
Janetzko J, Trauger SA, Lazarus MB, Walker S
Nat Chem Biol. 2016 Nov;12(11):899-901. doi: 10.1038/nchembio.2173. Epub 2016 Sep 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154279 | pBJG1 T7-8xHis-OGT D554N | Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site. |