Synthetic control devices for gene regulation in Penicillium chrysogenum.
Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 10.1186/s12934-019-1253-3.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154221 | pVE2_1_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_1xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 1xQUAS+CP_pcbC |
| 154222 | pLM2_30_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC |
| 154223 | pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 11xQUAS+CP_pcbC |
| 154224 | pLM2_33_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_pcbC (no QUAS control) |
| 154225 | pLM2_45_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS | Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter |
| 154226 | pVE2_12_p40S_QfDBD_VP16AD_5xQUAS_DsRed | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS (no CP control) |
| 154227 | pVE2_9_p40S_QfDBD_VP16AD_cp_pcbAB_DsRed_0xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_pcbAB (no QUAS control) |
| 154228 | pVE2_10_p40S_QfDBD_VP16AD_cp_penDE_DsRed_0xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_penDE (no QUAS control) |
| 154229 | pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control) |
| 154230 | pLM2_32_p40S_QfDBD_VP16AD_cp_nirA_DsRed_0xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_nirA (no QUAS control) |
| 154231 | pLM2_31_p40S_QfDBD_VP16AD_cp_ura3_DsRed_0xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_ura3 (no QUAS control) |
| 154232 | pVE2_5_p40S_QfDBD_VP16AD_cp_pcbAB_DsRed_5xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbAB |
| 154233 | pVE2_6_p40S_QfDBD_VP16AD_cp_penDE_DsRed_5xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_penDE |
| 154234 | pVE2_7_p40S_QfDBD_VP16AD_cp_phl_DsRed_5xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_phl |
| 154235 | pLM2_29_p40S_QfDBD_VP16AD_cp_nirA_DsRed_5xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_nirA |
| 154236 | pLM2_28_p40S_QfDBD_VP16AD_cp_ura3_DsRed_5xQUAS | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_ura3 |
| 154237 | pVE2_4_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS(rev) | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS(reversed)+CP_pcbC |
| 154238 | pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS | Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC |
| 154239 | pLM2_41_pgndA_QfDBD_VP16AD_cp_nirA_DsRed_5xQUAS | Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_nirA |
| 154240 | pLM2_40_pgndA_QfDBD_VP16AD_cp_ura3_DsRed_5xQUAS | Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_ura3 |
| 154241 | pVE2_19_p40S_QfDBD_VP16AD_pFullLength_pcbC_DsRed | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbC |
| 154242 | pVE2_20_p40S_QfDBD_VP16AD_pFullLength_pcbAB_DsRed | Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbAB |
| 154243 | pLM2_36_p40S_QfDBD_Qf2AD_cp_pcbC_DsRed_5xQUAS | Encodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC |
| 154244 | pLM2_35_p40S_QfDBD_Qf2AD_cp_nirA_DsRed_5xQUAS | Encodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_nirA |
| 154245 | pLM2_34_p40S_QfDBD_Qf2AD_cp_ura3_DsRed_5xQUAS | Encodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_ura3 |