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Dihydroxyacetone phosphate signals glucose availability to mTORC1.

Orozco JM, Krawczyk PA, Scaria SM, Cangelosi AL, Chan SH, Kunchok T, Lewis CA, Sabatini DM
Nat Metab. 2020 Sep;2(9):893-901. doi: 10.1038/s42255-020-0250-5. Epub 2020 Jul 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
154205HA-GLUT5 in pRK5N-terminal HA-tagged GLUT5 cDNA expression vector for transfections
154206FLAG-TKFC in pLJM60Lentiviral vector for stable expression of TKFC
154207FLAG-GK in PLJM60Lentiviral vector for stable expression of GK
154209TPI in pCW57_tTA_BlastDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI
1542103XFLAG_ALDOA in pCW57_tTA_BlastDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOA
1542113XFLAG_GAPDH in pCW57_tTA_BlastDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH
154212HA-mios in pRK5N-terminal HA-tagged Mios cDNA expression vector for transfections
154213WDR24 in pRK5Untagged WDR24 cDNA expression vector for transfections
154214WDR59 in pRK5Untagged WDR59 cDNA expression vector for transfections
154215SEC13 in pRK5Untagged SEC13 cDNA expression vector for transfections
154216SEH1L in pRK5Untagged SEH1L cDNA expression vector for transfections
154217FLAG-TKGAPDH in pLJM60Lentiviral vector for stable expression of FLAG-TK-GAPDH
154219HA_Axin1 in pRK5N-terminal HA-tagged AXIN1 cDNA expression vector for transfections
154220Axin1_HA in pRK5C-terminal HA-tagged AXIN1 cDNA expression vector for transfections

Antibodies from Article