Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters.
Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber J, Obenauf AC
Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 10.1038/s41587-020-0614-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154193 | dCas9-VPR_P2A_mCherry | Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker |
| 154194 | Dual-sgRNA_hU6-mU6 | Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker. |
| 154195 | CaTCH BC1 | Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1) |
| 154196 | CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C | Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker. |
| 154197 | CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C | Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker. |
| 157746 | CaTCH_empty-backbone | Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences |