LanCLs add glutathione to dehydroamino acids generated at phosphorylated sites in the proteome.
Lai KY, Galan SRG, Zeng Y, Zhou TH, He C, Raj R, Riedl J, Liu S, Chooi KP, Garg N, Zeng M, Jones LH, Hutchings GJ, Mohammed S, Nair SK, Chen J, Davis BG, van der Donk WA
Cell. 2021 Apr 27. pii: S0092-8674(21)00436-0. doi: 10.1016/j.cell.2021.04.001.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154186 | pETDuet1-WT-LanCL2-C-Ter-His | To express LanCL2 in bacterial cells |
| 154187 | pETDuet1-LanCL2-H264A-C-Ter-His | To express LanCL2-H264A in bacterial cells |
| 154188 | pETDuet1-LanCL2-C321A/C367A-C-Ter-His | To express LanCL2-C321A/C367A in bacterial cells |
| 154189 | pET-15b-LanCL1 | To express LanCL1 in bacterial cells |
| 154190 | pET-15b-LanCL1-H219A | To express LanCL1-H219A in bacterial cells |
| 154191 | pET-15b-LanCL1-C322A | To express LanCL1-C322A in bacterial cells |
| 154192 | pMAL-p2x-LanCL2 | To express MBP-LanCL2 in bacterial cells |