Engineering of Effector Domains for Targeted DNA Methylation with Reduced Off-Target Effects.
Hofacker D, Broche J, Laistner L, Adam S, Bashtrykov P, Jeltsch A
Int J Mol Sci. 2020 Jan 13;21(2). pii: ijms21020502. doi: 10.3390/ijms21020502.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154139 | dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP | Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation. |
| 154140 | scFv-GCN4-DNMT3a-DNMT3l | Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems. |
| 154141 | scFv-GCN4-DNMT3a(R887E)-DNMT3l | Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation. |
| 174141 | dCas9-10xSunTag-P2A-tagBFP | Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid. |