Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly.
Huang S, Aleksashin NA, Loveland AB, Klepacki D, Reier K, Kefi A, Szal T, Remme J, Jaeger L, Vazquez-Laslop N, Korostelev AA, Mankin AS
Nat Commun. 2020 Jun 9;11(1):2900. doi: 10.1038/s41467-020-16694-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154131 | pAM552 | Expresses wt rRNAs operon in bacterial cells |
| 154132 | pDH42(CUG/A) | Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells |
| 154133 | ptRNA100 | Expresses a set of tRNAs in bacterial cells |
| 155206 | SQ171/pCSacB/ptRNA100 | SQ171 strain supported by pCSacB and ptRNA100 plasmids |