A heat-shock inducible system for flexible gene expression in cereals.
Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, Richardson A
Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. eCollection 2020.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154056 | pNAM-B1 | Level 0 Golden Gate vector, contains domesticated NAM-B1 |
| 154057 | pZmUbi-5'UTR | Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct) |
| 154058 | pNB1-LOX | Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT |
| 154059 | pLOXp-GUS | Level 0.5 Golden Gate vector, loxP-GUS-nosT-loxP |
| 154060 | EC10161 | Level 0.5 Golden Gate backbone, containing loxP sites. |
| 154061 | EC71173 | Level 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-Cre |
| 154062 | EC71139 | Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct) |
| 154064 | HS_NAM-B1 | Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT |
| 154065 | EC71102 | Level 0 Golden Gate vector; Unmodifed CRE |
| 154066 | EC71167 | Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP |
| 154067 | EC71174 | Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER |
| 154068 | EC71100 | Level 0 Golden Gate vector, pHvHSP17 |
| 154069 | EC71171 | Level 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backbone |
| 159608 | EC71022 | Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP |