The Scaffold Protein Axin Promotes Signaling Specificity within the Wnt Pathway by Suppressing Competing Kinase Reactions.
Gavagan M, Fagnan E, Speltz EB, Zalatan JG
Cell Syst. 2020 Jun 24;10(6):515-525.e5. doi: 10.1016/j.cels.2020.05.002. Epub 2020 Jun 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154010 | pES001 MBP-GSK3β-HA-His | Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli |
| 154011 | pEF073 MBP-Axin-His | Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli |
| 154012 | pMG023 MBP-Axin 384-518-His (miniAxin) | Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli |
| 154053 | pMG035 MBP-Axin∆BCD-His | Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli |
| 154070 | pEF019 MBP-β-catenin-His | Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli |
| 154072 | pMG051 MBP-β-catenin-His, GST-CK1α | Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His |
| 154074 | pMG046 pETARA-CK1α-His | Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli |
| 154075 | pEF086 MBP-CREB 127-135-His | Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli |
| 154076 | pMG059 MBP-CREB-His | Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli |
| 154077 | pMG058 MBP-GS 634-666-His | Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli |