Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cells.
Chen Y, Liu J, Zhi S, Zheng Q, Ma W, Huang J, Liu Y, Liu D, Liang P, Songyang Z
Nat Commun. 2020 Jun 19;11(1):3136. doi: 10.1038/s41467-020-16880-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153942 | pCsy1-Csy2 | Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cells |
| 153943 | pCsy3-VPR-Csy4 | Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cells |
| 153944 | pCsy-crRNA-EV | For cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cells |