Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cells.

Chen Y, Liu J, Zhi S, Zheng Q, Ma W, Huang J, Liu Y, Liu D, Liang P, Songyang Z
Nat Commun. 2020 Jun 19;11(1):3136. doi: 10.1038/s41467-020-16880-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
153944	pCsy-crRNA-EV	For cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cells
153943	pCsy3-VPR-Csy4	Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cells
153942	pCsy1-Csy2	Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cells