Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53.
Hyodo T, Rahman ML, Karnan S, Ito T, Toyoda A, Ota A, Wahiduzzaman M, Tsuzuki S, Okada Y, Hosokawa Y, Konishi H
Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153530 | Donor-PIGA | Used as a donor vector for the targeted correction of a mutation in PIGA exon 6 |
| 153547 | Donor-PIGAshort | Used as a donor vector for the targeted correction of a mutation in PIGA exon 6 |
| 153548 | Donor-PIGAmut | Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6 |
| 153549 | Donor-PIGA-NeoR | Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette |
| 153551 | Donor-CD55-NeoR | Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette |
| 153958 | pCAG-EGxxFP-PIGA | Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences |
| 153959 | pCAG-EGxxFP-CD55 | Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences |
| 153960 | prCO | Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model) |
| 153961 | prCOrev | Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model) |
| 153962 | pMKO.1 puro-luc (shRNA) | Expresses a shRNA against firefly luciferase |
| 153966 | pEGFP-IREShygro2 | Expresses EGFP in mammalian cells |