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Quantitative live-cell imaging and computational modeling shed new light on endogenous WNT/CTNNB1 signaling dynamics.

de Man SM, Zwanenburg G, van der Wal T, Hink MA, van Amerongen R
Elife. 2021 Jun 30;10. pii: 66440. doi: 10.7554/eLife.66440. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
153429pX459-CTNNB1-ATGExpresses a gRNA that overlaps the startcodon of human CTNNB1
153430pRepair-SGFP2-CTNNB1Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2
153431pRepair-mScI-CTNNB1Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i
153432pRepair-SYFP2-CTNNB1Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2
153433pRepair-mTq2-CTNNB1Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2
164587pX459-CTNNB1-S45Expresses a gRNA that overlaps the S45 codon of human CTNNB1

Antibodies from Article