Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System.
Mann SE, Zhou Z, Landry LG, Anderson AM, Alkanani AK, Fischer J, Peakman M, Mallone R, Campbell K, Michels AW, Nakayama M
Front Immunol. 2020 Apr 9;11:633. doi: 10.3389/fimmu.2020.00633. eCollection 2020.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153415 | A2_uSFFV | Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter |
| 153417 | 8xNFAT-ZsG-hCD8 | NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene |
| 153418 | CD3-2A_pMI-LO | Retroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrange |
| 153423 | pMIII-Neo-mChe | Retroviral (MSCV) expression of mCherry fluorescent protein |
| 153424 | pMIII-Neo-tdTomato | Retroviral (MSCV) expression of tdTomato fluorescent protein |
| 153425 | pMIII-Neo-E2Crimson | Retroviral (MSCV) expression of E2-Crimson fluorescent protein |
| 153426 | pMIII-Neo-BFP | Retroviral (MSCV) expression of mTagBFP2 fluorescent protein |
| 153427 | pMIII-Neo-AM | Retroviral (MSCV) expression of ametrine fluorescent protein |
| 153428 | pMIII-Neo-LO | Retroviral (MSCV) expression of LSSmOrange fluorescent protein |