Directed evolution of VanR biosensor specificity in yeast
D'Ambrosio, V., Pramanik, S., Goroncy, K., Jakociunas, T., Schonauer, D., Davari, H. D., Schwaneberg, U., Keasling, J.D., Jensen, M.K.
Biotechnology Notes, Volume 1, 2020, Pages 9-15
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153339 | VanR-wt | Wild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoter |
| 158965 | VanR - F165V | Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter. |
| 158966 | VanR - F165R | Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter. |