Highly efficient multiplex editing: One-shot generation of 8x Nicotiana benthamiana and 12x Arabidopsis mutants.

Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
153228	pDGE347	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153229	pDGE651	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153230	pDGE652	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153231	pDGE666	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153232	pDGE667	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153233	pDGE668	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153234	pDGE463	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153235	pDGE669	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153236	pDGE670	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153237	pDGE671	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153238	pDGE672	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153239	pDGE673	Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
153240	pDGE331	M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153241	pDGE332	M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153242	pDGE333	M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153243	pDGE334	M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153244	pDGE335	M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153245	pDGE336	M4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153246	pDGE337	M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153247	pDGE495	M5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153248	pDGE496	M6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153249	pDGE497	M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153250	pDGE341	M7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153251	pDGE342	M8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153252	pDGE654	M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153253	pDGE655	M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153254	pDGE656	M2 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153255	pDGE657	M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153256	pDGE658	M3 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153257	pDGE659	M4 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153258	pDGE660	M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153259	pDGE661	M5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153260	pDGE662	M6 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153261	pDGE663	M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153262	pDGE664	M7 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153263	pDGE665	M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153264	pDGE473	M1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153265	pDGE474	M1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153266	pDGE475	M2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153267	pDGE476	M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153268	pDGE477	M3 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153269	pDGE478	M4 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153270	pDGE479	M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153271	pDGE498	M5 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153272	pDGE499	M6 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153273	pDGE500	M6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153274	pDGE501	M7 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153275	pDGE502	M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153276	pDGE503	Intermediate sgRNA array cloning vector position 1
153277	pDGE504	Intermediate sgRNA array cloning vector position 2
153278	pDGE505	Intermediate sgRNA array cloning vector position 3
153279	pDGE506	Intermediate sgRNA array cloning vector position 4
153280	pDGE508	End-linker for cloning 2 sgRNA arrays into any recipient vector
153281	pDGE509	End-linker for cloning 3 sgRNA arrays into any recipient vector
153282	pDGE510	End-linker for cloning 4 sgRNA arrays into any recipient vector