Highly efficient multiplex editing: One-shot generation of 8x Nicotiana benthamiana and 12x Arabidopsis mutants.

Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
153282	pDGE510	End-linker for cloning 4 sgRNA arrays into any recipient vector
153281	pDGE509	End-linker for cloning 3 sgRNA arrays into any recipient vector
153280	pDGE508	End-linker for cloning 2 sgRNA arrays into any recipient vector
153279	pDGE506	Intermediate sgRNA array cloning vector position 4
153278	pDGE505	Intermediate sgRNA array cloning vector position 3
153277	pDGE504	Intermediate sgRNA array cloning vector position 2
153276	pDGE503	Intermediate sgRNA array cloning vector position 1
153275	pDGE502	M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153274	pDGE501	M7 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
153273	pDGE500	M6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.