Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange.
Nonet ML
Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153083 | pLF3FShC | RMCE integration vector with self excising cassette |
| 153086 | pNM3732 | mec-4p GFP-C1 positive control for RMCE integration |
| 154108 | pNM3749 | loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector |
| 154109 | pNM3746 | loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector |
| 154110 | pNM3649 | miniMos dual component RMCE landing site vector |
| 154111 | pNM3689 | miniMos single component RMCE landing site vector |
| 154112 | pNM3724 | tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector |
| 154113 | pNM3725 | UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector |
| 154114 | pNM3726 | QUAS 5X ∆pes-10 promoter driving GFP-C1 in an RCME vector |
| 154115 | pNM3727 | lexO 5X ∆pes-10 promoter driving GFP-C1 in an RCME vector |
| 154116 | pNM3755 | tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector |
| 154117 | pNM3774 | tetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1 |
| 154118 | pNM3734 | mec-4 promoter driving GAL4-QFAD in an RCME vector |
| 154119 | pNM3810 | mec-4 promoter driving QF in an RCME vector |
| 154120 | pNM3811 | mec-4 promoter driving QF2 in an RCME vector |
| 154121 | pNM3813 | mec-4 promoter driving lexA-L-QFAD in an RCME vector |
| 154122 | pNM3814 | mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector |
| 154123 | pNM3823 | mec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vector |