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Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements.

Fontana J, Dong C, Kiattisewee C, Chavali VP, Tickman BI, Carothers JM, Zalatan JG
Nat Commun. 2020 Apr 1;11(1):1618. doi: 10.1038/s41467-020-15454-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
153024pCD442dCas9, MCP-SoxS(R93A/S101A)
153025pCK005.6dCas9, MCP-SoxS(R93A/S101A), J106 scRNA
153026pCD564dxCas9(3.7), MCP-SoxS(R93A/S101A)
153027pCD565dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
153028pJF143.J3J3-mRFP1

Antibodies from Article