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Facile assembly and fluorescence-based screening method for heterologous expression of biosynthetic pathways in fungi.

Hoefgen S, Lin J, Fricke J, Stroe MC, Mattern DJ, Kufs JE, Hortschansky P, Brakhage AA, Hoffmeister D, Valiante V
Metab Eng. 2018 Jul;48:44-51. doi: 10.1016/j.ymben.2018.05.014. Epub 2018 May 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
153022pV2A-TSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

Antibodies from Article