Split-TurboID enables contact-dependent proximity labeling in cells.
Cho KF, Branon TC, Rajeev S, Svinkina T, Udeshi ND, Thoudam T, Kwak C, Rhee HW, Lee IK, Carr SA, Ting AY
Proc Natl Acad Sci U S A. 2020 May 18. pii: 1919528117. doi: 10.1073/pnas.1919528117.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153002 | pLX304 CMV FKBP-V5-sTurboID (N) | Expresses low-affinity split-TurboID N-terminal fragment in mammalian cells |
| 153003 | pLX304 CMV HA-HaloTag-FRB-sTurboID (C) | Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells |
| 153004 | pLX304 CMV FKBP-V5-sTurboID-high affinity (N) | Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells |
| 153005 | pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C) | Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells |
| 153006 | pLX304 CMV OMM-FKBP-V5-sTurboID (N) | Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane |
| 153007 | pLX208 CMV sTurboID (C)-HA-FRB-ERM | Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane |
| 153008 | pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP | Expresses glucagon peptide on mammalian cell surface |
| 153009 | pAAV CMV GCGR-V5-sTurboID (N) | Expresses split-TuboID N-terminal fragment fused to GCGR |
| 153010 | pCDNA3 CMV sTurboID (C)-Arrestin-GFP | Expresses split-TurboID C-terminal fragment fused to arrestin |