A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi.
Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C
Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20.
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PubMed
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
118229 | pBSV2H | Empty E. coli - B. burgdorferi shuttle vector; hygromycin B resistant |
149557 | pKFSS1_Psyn-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149558 | pBSV2B_Psyn-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149559 | pBSV2H_Psyn-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149560 | pBSV2G_Psyn-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149561 | pBSV2H_Psyn-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149562 | pBSV2G_Psyn-sgRNAftsI | ftsI gRNA expression in B. burgdorferi |
149563 | pBSV2H_Psyn-sgRNAftsI | ftsI gRNA expression in B. burgdorferi |
149566 | pBSV2G_Psyn-sgRNAmreB | mreB gRNA expression in B. burgdorferi |
149567 | pBSV2H_Psyn-sgRNAmreB | mreB gRNA expression in B. burgdorferi |
149568 | pBSV2G_Psyn-sgRNArodA | rodA gRNA expression in B. burgdorferi |
149569 | pBSV2H_Psyn-sgRNArodA | rodA gRNA expression in B. burgdorferi |
149570 | pBbdCas9K_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149571 | pBbdCas9G_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149572 | pBbdCas9B_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149573 | pBbdCas9H_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149574 | pBbdCas9S(RBSmut) | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149575 | pBbdCas9S(RBSmut)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149576 | pBbdCas9S(RBSmut)_Psyn-sgRNA500 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149577 | pBbdCas9K(RBSmut)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149578 | pBbdCas9G(RBSmut)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149579 | pBbdCas9B(RBSmut)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149580 | pBbdCas9H(RBSmut)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149581 | pBbdCas9S(-10TC) | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149582 | pBbdCas9S(-10TC)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149583 | pBbdCas9S(-10TC)_Psyn-sgRNA500 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149584 | pBbdCas9K(-10TC)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149585 | pBbdCas9G(-10TC)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149586 | pBbdCas9B(-10TC)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149587 | pBbdCas9H(-10TC)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149588 | pBbdCas9S(RBSmut)_Psyn-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
149589 | pBbdCas9S(-10TC)_Psyn-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
149590 | pBbdCas9S(RBSmut)_Psyn-sgRNAftsI | all-in-one CRISPRi vector for targeting B. burgdorferi ftsI |
149591 | pBbdCas9S(-10TC)_Psyn-sgRNAftsI | all-in-all CRISPRi vector for targeting B. burgdorferi ftsI |
149594 | pBbdCas9S(RBSmut)_Psyn-sgRNAmreB | all-in-one CRISPRi vector for targeting B. burgdorferi mreB |
149595 | pBbdCas9S(-10TC)_Psyn-sgRNAmreB | all-in-one CRISPRi vector for targeting B. burgdorferi mreB |
149613 | pBSV2_Psyn-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149614 | pBSV2G_Psyn-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149615 | pBSV2G_PflaBS-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149616 | pBSV2G_PresTS-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149617 | pBSV2G_PresTL-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149618 | pBSV2G_P0826S-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149619 | pBSV2G_P0826L-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149620 | pBSV2G_P0026-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149621 | pBSV2G_P0526-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149622 | pBSV2G_P0031-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
149623 | pBSV2G_PflaBS-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149624 | pBSV2G_PresTS-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149625 | pBSV2G_PresTL-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149626 | pBSV2G_P0826S-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149627 | pBSV2G_P0826L-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149628 | pBSV2G_P0026-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149629 | pBSV2G_P0526-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149630 | pBSV2G_P0031-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149631 | pBSV2_Psyn-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
149632 | pBSV2_Psyn-sgRNAftsI | ftsI gRNA expression in B. burgdorferi |
149634 | pBSV2_Psyn-sgRNAmreB | mreB gRNA expression in B. burgdorferi |
149635 | pBSV2_Psyn-sgRNArodA | rodA gRNA expression in B. burgdorferi |
149636 | pBSV2_Psyn-mCherryBb | Psyn-mCherry promoter strength reporter for use in B. burgdorferi |
149637 | pKIKan_idCas9_Chr_center | suicide vector for knock in into the B. burgdorferi chromosome |
149638 | pBbdCas9S | parental, all-in-one CRISPRi vector for B. burgdorferi, empty |
149639 | pBbdCas9S_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149640 | pBbdCas9S_Psyn-sgRNA500 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149641 | pBbdCas9S_P0526-sgRNA500 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
149642 | pBbdCas9S_Psyn-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
149643 | pBbdCas9S_PflaBS-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
149644 | pBbdCas9S_PresTS-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
149645 | pBbdCas9S_PresTL-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
149646 | pBbdCas9S_P0026-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
149647 | pBbdCas9S_P0826S-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
149648 | pBbdCas9S_P0826L-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
149649 | pBbdCas9S_Psyn-sgRNAftsI | all-in-one CRISPRi vector for targeting B. burgdorferi ftsI |
149650 | pBbdCas9S_P0526-sgRNAftsI | all-in-one CRISPRi vector for targeting B. burgdorferi ftsI |
149653 | pBbdCas9S_Psyn-sgRNAmreB | all-in-one CRISPRi vector for targeting B. burgdorferi mreB |
149654 | pBbdCas9S_P0526-sgRNAmreB | all-in-one CRISPRi vector for targeting B. burgdorferi mreB |
149655 | pBbdCas9S_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
149656 | pBbdCas9S(RBSmut)_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
149657 | pBbdCas9S(-10TC)_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
149658 | pBbdCas9S_P0526-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
149659 | pBbdCas9S(-10AC1)_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
149660 | pBbdCas9S(-10AC2)_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
149661 | pBbdCas9S(-10AC12)_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |