Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences.
Sretenovic S, Yin D, Levav A, Selengut JD, Mount SM, Qi Y
Plant Commun. 2020 Jul 22;2(2):100101. doi: 10.1016/j.xplc.2020.100101. eCollection 2021 Mar 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149553 | pYPQ166-iSpyMac | Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs |
| 149554 | pYPQ265H | Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs |
| 149555 | pYPQ266B | Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs |
| 149556 | pYPQ262m3 | Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs |