Ginkgo Bioworks Lab: Truncated SARS-CoV-2 Spike protein constructs for making VSV pseudo virus
Unpublished
Codon optimization has been found to enhance expression of the Spike protein in lentiviral pseudovirons. Addgene plasmids 149539, 149541, and 149543 are the codon optimized versions from this article.
Please see Addgene's curated Ginkgo Bioworks COVID-19 Plasmid Collection page for additional information about the collection.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149539 | pGBW-m4137382 | Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping |
| 149540 | pGBW-m4137386 | Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein) |
| 149541 | pGBW-m4137383 | Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping |
| 149542 | pGBW-m4137385 | Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein) |
| 149543 | pGBW-m4137384 | Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping |
| 149544 | pGBW-m4137387 | Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein) |