A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background.

Budagyan K, Chernoff J
Methods Mol Biol. 2021;2262:323-334. doi: 10.1007/978-1-0716-1190-6_20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
149447	pLentiCas9-P2A-BFP	Expresses Cas9 and BFP in a lentiviral vector.
149448	U6::sgBFP-U6::sgKrasG12D-pX333-red	pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.