Temperature-Inducible Precision-Guided Sterile Insect Technique.
Kandul NP, Liu J, Akbari OS
CRISPR J. 2021 Dec;4(6):822-835. doi: 10.1089/crispr.2021.0077. Epub 2021 Nov 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149424 | 1137=Hsp70Bb-Cas9[dsRed] | The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene) |
| 149425 | 1046E=dgRNAs[traB,bTub] | The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes. |
| 149426 | 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9] | The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10. |
| 149427 | 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9] | The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10. |
| 153284 | 1137B=Hsp70Bb-Cas9 | The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker. |