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Identification of Plant Enhancers and Their Constituent Elements by STARR-seq in Tobacco Leaves.

Jores T, Tonnies J, Dorrity MW, Cuperus J, Fields S, Queitsch C
Plant Cell. 2020 May 14. pii: tpc.20.00155. doi: 10.1105/tpc.20.00155. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
149416pPSupPlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter.
149417pPSdownPlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the 3'-UTR of the GFP reporter gene.
149418pPSup2Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted about 1.4 kb upstream of the minimal promoter.
149419pPSdown2Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted downstream of the GFP reporter gene.
149420pPSup_iGFPPlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter. The GFP reporter gene harbors the IV2 intron.
149421pPSintPlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.
149422pPSup_35Senh(fwd)_35Spr+synJPlasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.
149423pZS*11_4enhPlasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

Antibodies from Article