Distribution and phasing of sequence motifs that facilitate CRISPR adaptation.
Santiago-Frangos A, Buyukyoruk M, Wiegand T, Krishna P, Wiedenheft B
Curr Biol. 2021 Aug 23;31(16):3515-3524.e6. doi: 10.1016/j.cub.2021.05.068. Epub 2021 Jun 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149384 | pHisIHFαIHFβ | Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta |
| 149385 | pStrepIHFb | Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta |
| 149386 | pCRISPR2_wt | P. aeruginosa PA14 CRISPR2 locus |
| 149387 | pCRISPR2_-6D | P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site |
| 149388 | pCRISPR2_-5D | P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site |
| 149389 | pCRISPR2_+1D | P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site |
| 149390 | pCRISPR2_+5D | P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site |
| 149391 | pCRISPR2_+10D | P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox site |
| 149393 | pCRISPR2_+10D+7U | P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site |
| 149394 | pCRISPR2_+10D+10U | P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site |
| 149395 | pCRISPR2_IHF_Opt | P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site |
| 149396 | pCRISPR2_IHF_Mut | P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF |
| 149397 | pCRISPR2_motif_scram | P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif |
| 162318 | pCRISPR2_IHFdist_rm | P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed |
| 162319 | pCRISPR2_IRdist_scram | P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif |