Creation of Golden Gate constructs for gene doctoring.
Thomson NM, Zhang C, Trampari E, Pallen MJ
BMC Biotechnol. 2020 Oct 7;20(1):54. doi: 10.1186/s12896-020-00648-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149377 | pDOC-GG | Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring |
| 149379 | mUAVbla:tetR 5′ | mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs |